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KMID : 0613820180280070772
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Journal of Life Science
2018 Volume.28 No. 7 p.772 ~ p.777
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Analysis of Antioxidant Enzyme Activity During Seedling Growth of Leymus chinensis Trin Under Salt and Dehydration Stresses
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Shim Dong-Hwan
Nam Ki-Jung
Kim Yun-Hee
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Abstract
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To understand the adaptability of Leymus chinensis forage grass to environmental stresses, we analyzed the H2O2 scavenging activity based on several antioxidant enzymes and total phenolics content, including peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT), in shoots and roots subjected to salt and dehydration stresses during seedling growth. After NaCl or PEG treatment, plants showed reduced seedling growth under over 200 mM NaCl or 30% PEG treatment condition in shoots and roots compared with the control condition. In addition, plants showed high enzymatic activity of CAT in the shoots, whereas they exhibited high activity levels of APX and POD in the roots in both the NaCl and PEG treatment conditions. These results seem to indicate that Leymus chinensis seedlings responding to salt and dehydration stresses during initial growth is associated with enhanced activity of H2O2 scavenging antioxidant enzymes in the shoots or roots. The plants also showed high levels of total phenolics under NaCl treatment, with a high concentration in both the shoots and roots. Our results showed that the induced activity patterns of APX in the roots and CAT in the shoots indicate that low H2O2 levels were mainly maintained through tissue-specific redox homeostasis involving enzymes such as APX and CAT during salt and dehydration stresses. This study highlights the importance of antioxidant enzymes in the establishment of Leymus chinensis seedlings under high salinity conditions, such as typical desertification.
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KEYWORD
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Antioxidant enzymes, drought, leymus chinensis, salt, seedling growth, total phenolics
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